TCEP hydrochloride, TCEP·HCl 99% CAS NO.51805-45-9

Tri (2-carboxyethyl) phosphine hydrochloride (TCEP · HCl) is a thiol free and odorless compound, which is widely used in biochemistry and molecular biology as a disulfide bond reducing agent for peptides or proteins. With other similar reducing agents such as dithiothreitol (DTT), β- Mercaptoethanol（ β- Compared with ME), TCEP · HCl has many advantages:

1) No odor - it can be used on the test bench without fume hood;

2) High stability - the stability of the structure of the compound itself makes it unnecessary to take any preventive measures to avoid oxidation during operation, use and preservation. It will not volatilize and can react at room temperature. Stable at room temperature, resistant to air oxidation. Stable in water-soluble buffer, acid and alkali. It will not react with other functional groups in the protein;

3) Wide pH working range - it is almost soluble in water soluble buffer of any pH value, and the effective working range of pH is 1.5-9.0. The pH value after directly dissolving in water is about 2.5;

4) Strong reducibility - For most experiments, 5-50 mM TCEP · HCl can provide enough molar force to fully reduce the disulfide bond by acting at room temperature for a few minutes;

5) Wide compatibility - it does not contain mercaptan group, so it is not necessary to remove this reductant in advance for downstream mercapto modification experiments such as maleimide coupling.

This product is suitable for the preparation steps of gel electrophoresis and solid phase metal ion affinity chromatography (IMAC). It is used as a reducing agent to destroy disulfide bonds within or between proteins. It is also particularly suitable for the reaction of maleimide coupling cysteine residues. It can prevent cysteine residues from forming disulfide bonds, and is not similar to DTT or β- ME itself is easy to react with maleimide. RNA isolation experiments are also commonly used in the tissue homogenate step.

Xuzhou Maikai Biotechnology Co., Ltd. was established in November 2021 with a registered capital of 15 million yuan. It is an emerging R&D enterprise based on anti-tumor drugs and biotechnology. The company cooperates with Dalian University of Technology, Zhejiang University, Jinan University, etc. Colleges and universities have good cooperative relations. The founders and shareholders of the company have 20 years of working experience in the chemical industry. They have in-depth understanding and control of the product market. The technology is mature and advanced, and the product quality has reached the pharmaceutical level, which has been fully recognized by customers in the industry.

The company focuses on the biochemical industry, aspires to become the world's leading brand in this industry, and contributes to the cause of human health. Since its establishment, the company has always adhered to and adhered to the core business philosophy of "morality-based, honesty-first", and regards providing value to customers as our responsibility, which has won praise from customers.

Pharmaceutical Division

We are mainly responsible for the R&D, production and sales of pharmaceutical intermediates and APIs. The products are used in the fields of bio-buffering agents, anti-tumor drugs, cardiovascular and cerebrovascular diseases, steroid drugs, etc.

Electronic New Materials Division

We are mainly engaged in the research and development of additives for the battery industry, such as fluorinated ethylene carbonate (FEC), chlorinated ethylene carbonate (CEC), etc. We deliver high quality and high specification products to the battery industry at home and abroad.

Custom Synthesis Division

We mainly undertake the development of products commissioned by domestic and foreign pharmaceutical companies and CDMO companies. The company has strong technical research on special types of reactions such as coupling, chlorination, diazotization, hydrogenation, and ammoniation. We are also currently working on product process improvement using continuous flow equipment to obtain faster and safer reaction conditions.

Pesticide Division

We are mainly responsible for the research and development, production and sales of intermediates and original drugs for plant growth regulator products.